Activation of primary sensory neurons by the topical application of capsaicin on the epidermis of a re-innervated organotypic human skin model.

@article{Lebonvallet2014ActivationOP,
  title={Activation of primary sensory neurons by the topical application of capsaicin on the epidermis of a re-innervated organotypic human skin model.},
  author={Nicolas Lebonvallet and Jean-Pierre Pennec and Christelle Le Gall-Ianotto and J{\'e}r{\'e}my Ch{\'e}ret and Christine Jeanmaire and J. Carre and Gilles Pauly and Laurent Misery},
  journal={Experimental dermatology},
  year={2014},
  volume={23 1},
  pages={
          73-5
        }
}
Using an ex vivo skin-nerve preparation, skin and nerve cells were reconstituted into a single unit and maintained in a nutrient medium bath until required experimentally. Our objective was to use the epidermis as a relay for the induction of an electric current to the neurons following the topical application of capsaicin on the skin epidermis of the skin explant, an agonist of the TRPV1 channel implicated in pruritus and pain. After 10-20 days of coculture to form the re-innervated skin model… CONTINUE READING