Activation of phospholipase A2 by the nociceptin receptor expressed in Chinese hamster ovary cells.

@article{Fukuda1998ActivationOP,
  title={Activation of phospholipase A2 by the nociceptin receptor expressed in Chinese hamster ovary cells.},
  author={Kazuhiko Fukuda and Takehiro Shoda and Hitoshi Morikawa and Shigeaki Kato and Hiroyuki Mima and Koichi Mori},
  journal={Journal of neurochemistry},
  year={1998},
  volume={71 5},
  pages={2186-92}
}
To gain insight into the molecular mechanism for nociceptin function, functional coupling of the nociceptin receptor expressed in Chinese hamster ovary (CHO) cells with phospholipase A2 (PLA2) was examined. In the presence of A23187, a calcium ionophore, activation of the nociceptin receptor induced time- and dose-dependent release of arachidonate, which was abolished by pretreatment of the cells with pertussis toxin (PTX). Immunoblot analysis using anti-Ca2+-dependent cytosolic PLA2 (cPLA2… CONTINUE READING