Activation of iron regulatory protein-1 by oxidative stress in vitro.

  title={Activation of iron regulatory protein-1 by oxidative stress in vitro.},
  author={Kostas Pantopoulos and Matthias W. Hentze},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={95 18},
Iron regulatory protein-1 (IRP-1), a central cytoplasmic regulator of cellular iron metabolism, is rapidly activated by oxidative stress to bind to mRNA iron-responsive elements. We have reconstituted the response of IRP-1 to extracellular H2O2 in a system derived from murine B6 fibroblasts permeabilized with streptolysin-O. This procedure allows separation of the cytosol from the remainder of the cells (cell pellet). IRP-1 in the cytosolic fraction fails to be directly activated by addition of… CONTINUE READING
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