Corpus ID: 14971700

Activation of inositol phospholipid signaling and Ca2+ efflux in human breast cancer cells by bombesin.

@article{Patel1990ActivationOI,
  title={Activation of inositol phospholipid signaling and Ca2+ efflux in human breast cancer cells by bombesin.},
  author={K. V. Patel and Michael P. Schrey},
  journal={Cancer research},
  year={1990},
  volume={50 2},
  pages={
          235-9
        }
}
Members of the bombesin-related family of peptides (BRPs) are mitogenic for a variety of cell types; however, a role for these peptides has not been previously described in human breast cancer. Early membrane receptor signal transduction mechanisms associated with bombesin action include phospholipase C-mediated inositol phospholipid hydrolysis and the elevation of cytosolic Ca2+ levels. We have investigated a potential role for BRPs in breast cancer by studying their effect on phospholipid… Expand
Evidence for a role for protein kinase C in the modulation of bombesin-activated cellular signalling in human breast cancer cells
TLDR
Data support an inhibitory role for PKC in the regulation of phosphoinositide hydrolysis and [Ca2+]i in breast cancer cells and provide a potential mechanism for feedback regulation of this signalling pathway in these cells. Expand
Bombesin and glucocorticoids stimulate human breast cancer cells to produce endothelin, a paracrine mitogen for breast stromal cells.
TLDR
In vitro study demonstrates the potential for bombesin and glucocorticoid to regulate ET production in human breast cancer cells, which may in turn have a paracrine influence on neighboring stromal cell function. Expand
Heterologous regulation of inositol lipid hydrolysis in human breast cancer cells by oestradiol 17β, bombesin and fluoroaluminate
TLDR
The potentiation by oestradiol of both bombesin‐ and AIF4−‐stimulated inositol lipid hydrolysis suggests the operation of a post‐receptor regulatory mechanism(s) which is independent of the inositl lipid pool size. Expand
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TLDR
The results are in favour of a role of IICS in MCF-7 cell growth, and it is hypothesized that the regulation of IP3R3 expression by E2 is involved in this effect. Expand
Advances in Brief Stimulation by Bombesin and Inhibition by Bombesin / Gastrin-releasing Peptide Antagonist RC-3095 of Growth of Human Breast Cancer Cell Lines 1
Recently, it was reported that bombesin/gastrin-releasing peptide (GRP) have mitogenic effects on some human breast cancer cell lines. In this study, we investigated the effects of bombesin/GRP andExpand
Inhibition of DNA Synthesis and Growth in Human Breast Stromal Cells by Bradykinin : Evidence for Independent Roles of BI and B 2 Receptors in the Respective Control of Cell Growth and Phospholipid Hydrolysis 1
The paracrine and intracellular mechanisms controlling stromal cell growth in the normal or neoplastic breast are unknown. This in vitro study uses human breast fibroblasts to investigate a potentialExpand
Stimulation of natural killer and antibody-dependent cellular cytotoxicity activities in mouse leukocytes by bombesin, gastrin-releasing peptide and neuromedin C: Involvement of cyclic AMP, inositol 1,4,5-trisphosphate and protein kinase C
TLDR
It is suggested that inositol phospholipid signalling and cAMP messenger systems are involved in the increase of NK and ADCC activities when leukocytes are incubated in the presence of bombesin, GRP or neuromedin C. Expand
Characterization of bombesin/gastrin-releasing peptide receptors in human breast cancer and their relationship to steroid receptor expression.
TLDR
Estimation of specific receptor proteins for BN/GRP, potent mitogens, in a large number of human breast cancers suggests that they may be involved in tumor cell progression and the approach based on determination of BN-GRP receptors might be useful to guide a hormonal therapy with BN /GRP antagonists in some women with breast cancer. Expand
Relationship of growth stimulated by lithium, estradiol, and EGF to phospholipase C activity in MCF-7 human breast cancer cells
TLDR
Results suggest that PLC-mediated PtdIns(4,5)P2 hydrolysis is not primarily associated with signaling proliferation by lithium or EGF in MCF-7 breast cancer cells. Expand
Bombesin stimulates proliferation of human breast cancer cells in culture.
TLDR
Recently, it was shown that both bombesin and GRP stimulate inositol phospholipid hydrolysis and Ca2" efflux in MCF-7 and T47D human breast cancer cells suggesting a role in mitogenic signalling, which suggests that a BLI-autocrine stimulatory loop may operate in some breast cancer cell lines such as SCCL cells. Expand
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References

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A novel bombesin receptor antagonist inhibits autocrine signals in a small cell lung carcinoma cell line.
TLDR
In conclusion, inhibition of transmembrane signals associated with autocrine growth control in SCLC by a novel peptide receptor antagonist is demonstrated. Expand
Modulation of bombesin-induced phosphatidylinositol hydrolysis in a small-cell lung-cancer cell line.
TLDR
The activation of phospholipase C in SCLC by bombesin congeners is demonstrated, and a regulatory role for protein kinase C, a cholera-toxin substrate, and perhaps a pertussis-t toxin substrate are suggested in the response of SclC tobombesin. Expand
Bombesin-like peptides elevate cytosolic calcium in small cell lung cancer cells.
TLDR
The data suggest that bombesin receptors may regulate the release of Ca2+ from intracellular organelles in small cell lung cancer cells. Expand
The effects of bombesin on polyphosphoinositide and calcium metabolism in Swiss 3T3 cells.
Bombesin, a peptide mitogen for a variety of cell types, acts as a typical Ca2+-mobilizing hormone in Swiss 3T3 fibroblasts. At its mitogenic concentrations (1-25 nM), bombesin stimulatesExpand
Bombesin-like peptides can function as autocrine growth factors in human small-cell lung cancer
TLDR
It is demonstrated that a monoclonal antibody to bombesin binds to the C-terminal region of BLPs, blocks the binding of the hormone to cellular receptors and inhibits the clonal growth of SCLC in vitro and the growth ofSCLC xenografts in vivo. Expand
The biphasic stimulation of insulin secretion by bombesin involves both cytosolic free calcium and protein kinase C.
TLDR
The data indicate that the initial secretory burst induced by bombesin results from the synergistic action of the high [Ca2+]i produced by InsP3 and DAG-activated protein kinase C, which appears to be sufficient for a sustained secretory response. Expand
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: Normal cells require growth factors to multiply. One group of growth factors such as platelet-derived growth factor, bombesin and vasopressin in fibroblasts or antigen in lymphocytes uses aExpand
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TLDR
It is demonstrated that hormones and growth factors which cross-regulate EGF receptor affinity in Swiss 3T3 cells have a common ability to stimulate the breakdown of phosphoinositides in these cells, and it is proposed that the reduction of EGF receptors affinity is a consequence of the activation of protein kinase C by the diacylglycerol generated by this breakdown. Expand
Tyrosine kinase-activating growth factors potentiate thrombin- and AIF4- -induced phosphoinositide breakdown in hamster fibroblasts. Evidence for positive cross-talk between the two mitogenic signaling pathways.
TLDR
Comparison of the potentiating effects of FGF on inositol phosphate formation and on DNA synthesis suggests than an increased production of second messengers by the inositl lipid pathway in the first hours of stimulation might be, at at least in part, responsible for the synergistic actions of F GF and thrombin onDNA synthesis. Expand
Bombesin stimulation of DNA synthesis and cell division in cultures of Swiss 3T3 cells.
TLDR
Bombesin and litorin may provide useful model peptides for studies on the mechanism(s) by which extracellular ligands control cell proliferation and vasopressin, phorbol esters, or cAMP increasing agents fail to enhance the maximal level of DNA synthesis induced by bombesin. Expand
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