Activation of human neutrophil procollagenase by nitrogen dioxide and peroxynitrite: a novel mechanism for procollagenase activation involving nitric oxide.

@article{Okamoto1997ActivationOH,
  title={Activation of human neutrophil procollagenase by nitrogen dioxide and peroxynitrite: a novel mechanism for procollagenase activation involving nitric oxide.},
  author={Tatsuya Okamoto and Takaaki Akaike and Takayuki Nagano and Sawako Miyajima and Moritaka Suga and Masayuki Ando and Koji Ichimori and Hidekatsu Maeda},
  journal={Archives of biochemistry and biophysics},
  year={1997},
  volume={342 2},
  pages={261-74}
}
The involvement of nitric oxide (NO) and its reactive intermediates such as nitrogen dioxide (NO2) and peroxynitrite (ONOO-) in the activation of matrix metallo-proteinase was investigated. The human neutrophil procollagenase (matrix metalloproteinase-8) (M(r), 85 kDa) was purified to homogeneity from human neutrophils by using column chromatography. After incubation of human neutrophil procollagenase with various nitrogen oxide-generating systems, collagenolytic activity in each reaction… CONTINUE READING