Activation of homologous recombination DNA repair in human skin fibroblasts continuously exposed to X-ray radiation

@inproceedings{Osipov2015ActivationOH,
  title={Activation of homologous recombination DNA repair in human skin fibroblasts continuously exposed to X-ray radiation},
  author={Andreyan N. Osipov and A. K. Grekhova and Margarita V Pustovalova and Ivan V. Ozerov and Petr Eremin and Natalia Vorobyeva and Natalia Lazareva and Andrey Pulin and Alex Zhavoronkov and Sergey A. Roumiantsev and Dmitry Y. Klokov and Ilya I Eremin},
  booktitle={Oncotarget},
  year={2015}
}
Molecular and cellular responses to protracted ionizing radiation exposures are poorly understood. Using immunofluorescence microscopy, we studied the kinetics of DNA repair foci formation in normal human fibroblasts exposed to X-rays at a dose rate of 4.5 mGy/min for up to 6 h. We showed that both the number of γH2AX foci and their integral fluorescence intensity grew linearly with time of irradiation up to 2 h. A plateau was observed between 2 and 6 h of exposure, indicating a state of… CONTINUE READING