Activation of a novel human transforming gene, ret, by DNA rearrangement

@article{Takahashi1985ActivationOA,
  title={Activation of a novel human transforming gene, ret, by DNA rearrangement},
  author={Masahide Takahashi and Jerome Ritz and Geoffrey M. Cooper},
  journal={Cell},
  year={1985},
  volume={42},
  pages={581-588}
}
Activation of human raf transforming genes by deletion of normal amino-terminal coding sequences
TLDR
Cl cloning sequences upstream of the truncated raf loci are shown to involve the fusion of three different 5' non-raf human sequences to the human raf gene, suggesting that 5' truncation alone may be sufficient to activate the transforming potential of cellular raf genes.
ret transforming gene encodes a fusion protein homologous to tyrosine kinases.
TLDR
The ret transforming gene was activated by recombination between two unlinked segments of human DNA, most likely during transfection of NIH 3T3 cells, and encoded a fusion protein with a carboxy-terminal domain which is 40 to 50% homologous to members of the tyrosine kinase gene family.
ret transforming gene encodes a fusion protein homologous to tyrosine kinases
TLDR
The ret transforming gene was activated by recombination between two unlinked segments of human DNA, most likely during transfection of NIH 3T3 cells, and encoded a fusion protein with a carboxy-terminal domain which is 40 to 50% homologous to members of the tyrosine kinase gene family.
Activation by Point Mutation of Ki-ras Gene Occurring in Transfected Human Normal dna
TLDR
A mutation-activated human Ki-ras gene was detected in NIH 3T3 cells transfected with high molecular weight DNA extracted from peripheral blood leukocytes of a healthy blood donor, and hypothesize that a base substitution occurred during the transfection assay.
Transforming DNA sequences present in human prolactin-secreting pituitary tumors.
TLDR
Five human PRL-secreting pituitary tumors were tested for the presence of DNA-transforming sequences and amplification of an Alu-containing DNA region within these transformants revealed that it contained sequences from the human hst gene, a member of the fibroblast growth factor family.
Molecular cloning and characterization of an activated human c-raf-1 gene
TLDR
Results of previous studies have shown that a raf-related transforming DNA sequence is present in NIH 3T3 transformants that are derived from GL-5-JCK human glioblastoma DNA transfection, but no rearrangement of c-raf-1 was detectable in the tumor DNA.
Molecular cloning and characterization of an activated human c-raf-1 gene.
TLDR
Results of previous studies have shown that a raf-related transforming DNA sequence is present in NIH 3T3 transformants that are derived from GL-5-JCK human glioblastoma DNA transfection, but no rearrangement of c-raf-1 was detectable in the tumor DNA.
Characterization of two new human oncogenes.
TLDR
The development of techniques for DNA transfer in eukaryotic cells led to the discovery of cellular transforming genes in tumor cells by their ability to induce foci of transformed NIH-3T3 cells.
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References

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TLDR
DNA from the human neuroblastoma cell line SK-N-SH is capable of inducing foci of transformed NIH 3T3 cells after DNA-mediated gene transfer and all three transforming gene sequences may be related members of the ras gene family.
Transformation of human cells by DNAs ineffective in transformation of NIH 3T3 cells.
TLDR
The results indicate that the human cell transfection system allows detection of transforming genes not effective in the 3T3 system and points out the possibility of detection of additional transforming sequences even in DNAs that do transform murine cells.
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TLDR
It seems that endogenous transforming genes of uninfected cells can be activated and efficiently transmitted by transfection, consistent with the hypothesis that normal cells contain genes that are capable of inducing transformation if expressed at abnormal levels.
Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change
TLDR
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TLDR
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TLDR
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A molecular clone containing part of the transforming gene from two human sarcoma cell lines, HT1080 and RD, has been obtained and shown to represent a new member of the human ras gene family. The
Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells
TLDR
It is found that T24, a cell line derived from a human bladder carcinoma, can induce the morphological transformation of NIH 3T3 cells, and the gene responsible for this transformation is human in origin, <5 kilobase pairs in size and homologous to a 1,100-base polyadenylated RNA species found in T24 and HeLa cells.
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