Activation of Xenopus eggs by the kinase inhibitor 6-DMAP suggests a differential regulation of cyclin B and p39(mos) proteolysis.

@article{Bodart1999ActivationOX,
  title={Activation of Xenopus eggs by the kinase inhibitor 6-DMAP suggests a differential regulation of cyclin B and p39(mos) proteolysis.},
  author={J F Bodart and D. Bechard and M Bertout and Julian Gannon and Adrien Rousseau and Jean Pierre Vilain and St{\'e}phane Flament},
  journal={Experimental cell research},
  year={1999},
  volume={253 2},
  pages={413-21}
}
In Xenopus eggs, metaphase II arrest is due to the cytostatic factor that maintains a high level of MPF activity. Kinases are important in this phenomenon since p39(mos) and MAPK play a part in the cytostatic activity whereas p34(cdc2) is the catalytic subunit of MPF. Fertilization induces a rise in intracellular calcium leading to egg activation that can be mimicked by calcium-increasing agents such as calcium ionophore. We have performed on Xenopus eggs a biochemical comparison of the effects… CONTINUE READING