Activation of Rho-dependent transcription termination by NusG. Dependence on terminator location and acceleration of RNA release.

@article{Burns1999ActivationOR,
  title={Activation of Rho-dependent transcription termination by NusG. Dependence on terminator location and acceleration of RNA release.},
  author={Christopher M. Burns and W L Nowatzke and John P. Richardson},
  journal={The Journal of biological chemistry},
  year={1999},
  volume={274 8},
  pages={5245-51}
}
There is a kinetic limitation to Rho function at the first intragenic terminator in the lacZ gene (tiZ1) which can be overcome by NusG: Rho can terminate transcription with slowly moving, but not rapidly moving, RNA polymerase unless NusG is also present. Here we report further studies with two other Rho-dependent terminators that are not kinetically limited (tiZ2 and lambda tR1) which show that the requirement for NusG depends on the properties of the terminator and its location in the… CONTINUE READING
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