Activation of Klebsiella pneumoniae and Rhizobium meliloti nitrogenase promoters by gln (ntr) regulatory proteins.

Abstract

We have studied the expression, in different Escherichia coli gln (ntr) mutants, of fusions (constructed in vitro) of the nifHDK (nitrogenase) promoters from Klebsiella pneumoniae and Rhizobium meliloti to E. coli lacZ. Derepression of the K. pneumoniae nifH::lacZ fusion requires the glnF (ntrA) gene product in addition to the K. pneumoniae nifA gene product, indicating that regulation of the K. pneumoniae nif genes is more closely integrated with the overall nitrogen control system than previously demonstrated. Derepression of the R. meliloti nifH::lacZ fusion in E. coli by the K. pneumoniae nifA gene product (which we had previously shown) exhibits the same requirement for glnF. Derepression of the R. meliloti nifH::lacZ fusion, but not the K. pneumoniae nifH::lacZ fusion, can be mediated by the glnG (ntrC) gene product, suggesting that the gln regulatory genes might directly regulate the symbiotic nitrogen fixation genes in Rhizobium.

Statistics

050100'85'88'91'94'97'00'03'06'09'12'15
Citations per Year

120 Citations

Semantic Scholar estimates that this publication has 120 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Sundaresan1983ActivationOK, title={Activation of Klebsiella pneumoniae and Rhizobium meliloti nitrogenase promoters by gln (ntr) regulatory proteins.}, author={Vinoth Sundaresan and David Ow and Frederick M. Ausubel}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={1983}, volume={80 13}, pages={4030-4} }