Activation of CPP32-like protease in tumor necrosis factor-induced apoptosis is dependent on mitochondrial function.

@article{Higuchi1997ActivationOC,
  title={Activation of CPP32-like protease in tumor necrosis factor-induced apoptosis is dependent on mitochondrial function.},
  author={Masahiro Higuchi and Bharat Bhushan Aggarwal and Edward T. H. Yeh},
  journal={The Journal of clinical investigation},
  year={1997},
  volume={99 7},
  pages={1751-8}
}
Mitochondria have been implicated in apoptosis, however, the precise mechanisms whereby mitochondria exert their effect are not clear. To gain further insights, we generated a panel of cells from ML-1a cells that were rendered respiration deficient by ethidium bromide treatment. Two respiration-deficient clones were subsequently reconstituted by fusion with platelets. Respiration-deficient clones were resistant to TNF-induced apoptosis, whereas ML-1a and reconstituted clones were sensitive. In… CONTINUE READING