Activation of Arabidopsis MAPK kinase kinase (AtMEKK1) and induction of AtMEKK1–AtMEK1 pathway by wounding

@article{Hadiarto2005ActivationOA,
  title={Activation of Arabidopsis MAPK kinase kinase (AtMEKK1) and induction of AtMEKK1–AtMEK1 pathway by wounding},
  author={Toto Hadiarto and Takashi Nanmori and Daisuke Matsuoka and Tetsushi Iwasaki and Ken-Ichi Sato and Yasuo Fukami and Tetsushi Azuma and Takeshi Yasuda},
  journal={Planta},
  year={2005},
  volume={223},
  pages={708-713}
}
We have constructed a series of deletion mutants of Arabidopsis MAPK kinase kinase (AtMEKK1) and obtained a constitutively active mutant, AtMEKK1Δ166, which lacks in self-inhibitory sequence of N-terminal 166 amino acids but still has substrate specificity. AtMEKK1Δ166 predominantly phosphorylates AtMEK1, an Arabidopsis MAPKK, but not its double mutant (AtMEK1T218A/S224E), suggesting that Thr-218 and Ser-224 are the phosphorylation sites. In wounded seedlings, AtMEKK1 was activated and… 
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