Activation of ADP-ribosyltransferase in polyamine-depleted mammalian cells.

  title={Activation of ADP-ribosyltransferase in polyamine-depleted mammalian cells.},
  author={Heather M. Wallace and Albert M. Gordon and H M Keir and Colin K. Pearson},
  journal={The Biochemical journal},
  volume={219 1},
Mammalian fibroblasts were cultured in the presence of alpha-methylornithine and/or methylglyoxal bis(guanylhydrazone), which inhibit the synthesis of polyamines. This led to a decrease in the cellular content of the polyamines spermine and spermidine by up to 60% when the cells were grown in the presence of both drugs together. The activity of the chromatin-associated enzyme ADP-ribosyltransferase was enhanced 2-3-fold in the drug-treated cells when measured in cells subsequently rendered… CONTINUE READING

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