Activation loop phosphorylation-independent kinase activity of human protein kinase C zeta.

@article{Ranganathan2007ActivationLP,
  title={Activation loop phosphorylation-independent kinase activity of human protein kinase C zeta.},
  author={Senthil Ranganathan and Yimin Wang and Francis G. Kern and Zhican Qu and Rongbao Li},
  journal={Proteins},
  year={2007},
  volume={67 3},
  pages={709-19}
}
Atypical protein kinase C zeta (PKCzeta) plays an important role in cell proliferation and survival. PKCzeta and its truncated form containing only the kinase domain, CATzeta, have been reported to be activated by the phosphorylation of threonine 410 in the activation loop. We expressed both the full length PKCzeta and CATzeta in a baculovirus/insect cell over-expression system and purified the proteins for biochemical characterization. Ion exchange chromatography of CATzeta revealed three… CONTINUE READING