Activation during preparation of therapeutic platelets affects deterioration during storage: a comparative flow cytometric study of different production methods.

@article{Metcalfe1997ActivationDP,
  title={Activation during preparation of therapeutic platelets affects deterioration during storage: a comparative flow cytometric study of different production methods.},
  author={Paul Metcalfe and Lorna M. Williamson and Chris P M Reutelingsperger and Ian J Swann and Willem H Ouwehand and Alison H. Goodall},
  journal={British journal of haematology},
  year={1997},
  volume={98 1},
  pages={86-95}
}
Three different separation methods, all using centrifugation, are routinely used to prepare therapeutic platelet concentrates from human donor blood. Platelet concentrates derived from platelet-rich plasma (PRP-PC), buffy coat (BC-PC) and apheresis (AP-PC) were investigated at the end of production, and over an 8 d storage period. Change in platelet surface markers were measured by flow cytometry, using fluorescein-conjugated antibodies to fibrinogen, P-selectin (CD62P), GPIIb-IIIa (CD41), GPIb… CONTINUE READING

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