Activation defects caused by mutations in Escherichia coli rpoA are promoter specific.

@article{Gussin1992ActivationDC,
  title={Activation defects caused by mutations in Escherichia coli rpoA are promoter specific.},
  author={Gary N. Gussin and Craig M. Olson and Kazuo Igarashi and Akira Ishihama},
  journal={Journal of bacteriology},
  year={1992},
  volume={174 15},
  pages={
          5156-60
        }
}
Escherichia coli RNA polymerases containing mutated alpha subunits were tested for their ability to respond to three different positive regulators (activators) in vitro. The two alpha (rpoA) mutants, alpha-256 and alpha-235, have deletions of the C-terminal 73 and 94 amino acids, respectively. In runoff transcription assays catalyzed by reconstituted holoenzyme, the effects of the mutations on each of three promoters tested were different: activation of the lambda pRM promoter by cI protein… CONTINUE READING
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