Activation by P 2 X 7 Agonists of Two Phospholipases A 2 ( PLA 2 ) in Ductal Cells of Rat Submandibular Gland

@inproceedings{Alzola1998ActivationBP,
  title={Activation by P 2 X 7 Agonists of Two Phospholipases A 2 ( PLA 2 ) in Ductal Cells of Rat Submandibular Gland},
  author={Eduardo Alzola and Arantza P{\'e}rez-Etxebarria and Elie Kabr{\'e} and David J. Fogarty and Mourad M{\'e}tioui and Naima Chaı̈b and Jos{\'e} Mar{\'i}a Macarulla and Carlos Matute and J. P. Dehaye and Aida Marino},
  year={1998}
}
Isolated ductal cells of rat submandibular gland phospholipid pools were labeled with [H]arachidonic acid (AA). The tracer was incorporated preferentially to phosphatidylcholine (46% of the lipidic fraction). Extracellular ATP induced the release of [H]AA to the extracellular medium in a timeand dose-dependent manner (EC50 5 220 mM). Among other agents tested, only 2*,3*O-(4-benzoylbenzoyl)adenosine 5*-triphosphate (BzATP) was able to mimic the effect of ATP (EC50 5 15 mM), without activation… CONTINUE READING