Activation and inhibition of rubber transferases by metal cofactors and pyrophosphate substrates.

@article{Scott2003ActivationAI,
  title={Activation and inhibition of rubber transferases by metal cofactors and pyrophosphate substrates.},
  author={Deborah J. Scott and Bernardo M T da Costa and Stephanie C Espy and Jay D Keasling and Katrina Cornish},
  journal={Phytochemistry},
  year={2003},
  volume={64 1},
  pages={123-34}
}
Metal cofactors are necessary for the activity of alkylation by prenyl transfer in enzyme-catalyzed reactions. Rubber transferase (RuT, a cis-prenyl transferase) associated with purified rubber particles from Hevea brasiliensis, Parthenium argentatum and Ficus elastica can use magnesium and manganese interchangably to achieve maximum velocity. We define the concentration of activator required for maximum velocity as [A](max). The [A](max)(Mg2+) in F. elastica (100 mM) is 10 times the [A](max… CONTINUE READING