Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074

@article{Olano2014ActivationAI,
  title={Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074},
  author={C. Olano and I. Garc{\'i}a and Ar{\'a}nzazu Gonz{\'a}lez and Miriam Rodr{\'i}guez and D. Rozas and J. Rubio and M. S{\'a}nchez-Hidalgo and A. Bra{\~n}a and C. M{\'e}ndez and J. Salas},
  journal={Microbial Biotechnology},
  year={2014},
  volume={7},
  pages={242 - 256}
}
Streptomyces albus J1074 is a streptomycete strain widely used as a host for expression of secondary metabolite gene clusters. [...] Key Result By insertion of a strong and constitutive promoter in front of selected genes of two clusters, production of the blue pigment indigoidine and of two novel members of the polycyclic tetramate macrolactam family (6-epi-alteramides A and B) was activated.Expand
Identification of butenolide regulatory system controlling secondary metabolism in Streptomyces albus J1074
TLDR
The application of a reporter-guided screening strategy to activate cryptic polycyclic tetramate macrolactam gene clusters in Streptomyces albus J1074 results in the selection of two S. albus strains with altered secondary metabolites production. Expand
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Production of a Novel Amide‐Containing Polyene by Activating a Cryptic Biosynthetic Gene Cluster in Streptomyces sp. MSC090213JE08
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Engineering of Streptomyces albus J1074 and Streptomyces lividans TK24 for natural products production
7 Abstract Actinobacteria have remarkable chemical potential that is not explored due to low level of corresponding genes expression. In order to uncover this reservoir of natural products weExpand
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Rational engineering of Streptomyces albus J1074 yielded a series of mutants with improved capabilities for native and heterologous expression of secondary metabolite gene clusters, and led to the activation of the native paulomycin pathway in engineered S. albus strains and importantly the upregulated expression of the heterologicous actinorhodin gene cluster. Expand
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Bioinformatic analysis of the genome of this organism predicts the presence of 38 putative secondary‐metabolite biosynthesis gene clusters, including those involved in the biosynthesis of AJI9561 and its derivative nataxazole, the antibiotic hygromycin B, and ionophores enterobactin and coelibactin. Expand
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