Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074

  title={Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074},
  author={Carlos Olano and Ignacio Garc{\'i}a and Ar{\'a}nzazu Gonz{\'a}lez and Miriam Rodr{\'i}guez and Daniel Rozas and Julio Rubio and Marina S{\'a}nchez-Hidalgo and Alfredo F Bra{\~n}a and Carmen M{\'e}ndez and Jos{\'e} A Salas},
  journal={Microbial Biotechnology},
  pages={242 - 256}
Streptomyces albus J1074 is a streptomycete strain widely used as a host for expression of secondary metabolite gene clusters. [] Key Result By insertion of a strong and constitutive promoter in front of selected genes of two clusters, production of the blue pigment indigoidine and of two novel members of the polycyclic tetramate macrolactam family (6-epi-alteramides A and B) was activated.

Identification of butenolide regulatory system controlling secondary metabolism in Streptomyces albus J1074

The application of a reporter-guided screening strategy to activate cryptic polycyclic tetramate macrolactam gene clusters in Streptomyces albus J1074 results in the selection of two S. albus strains with altered secondary metabolites production.

Activation of Secondary Metabolite Gene Clusters in Streptomyces clavuligerus by the PimM Regulator of Streptomyces natalensis

It is shown that constitutive expression of pimM in Streptomyces clavuligerus ATCC 27064 significantly affected its transcriptome and modifies secondary metabolism, resulting in deep metabolic changes.

Production of a Novel Amide‐Containing Polyene by Activating a Cryptic Biosynthetic Gene Cluster in Streptomyces sp. MSC090213JE08

It is thought that a new subfamily of type II polyketide synthase is involved in the biosynthesis of the polyene structure of ishigamide, which seems to have more than 20 cryptic biosynthetic gene clusters for secondary metabolites in Streptomyces sp.

Engineering of Streptomyces albus J1074 and Streptomyces lividans TK24 for natural products production

A reporter-guided screening strategy combined with transposon mutagenesis was used to activate silent polycyclic tetramate macrolactam biosynthesis gene cluster in Streptomyces albus J1074, leading to identification of new regulatory system consisting of transcriptional regulator XNR_3174 and bacterial hormone-like compound butenolide.

Rational engineering of Streptomyces albus J1074 for the overexpression of secondary metabolite gene clusters

Rational engineering of Streptomyces albus J1074 yielded a series of mutants with improved capabilities for native and heterologous expression of secondary metabolite gene clusters, and led to the activation of the native paulomycin pathway in engineered S. albus strains and importantly the upregulated expression of the heterologicous actinorhodin gene cluster.

Activation and mechanism of a cryptic oviedomycin gene cluster via the disruption of a global regulatory gene, adpA, in Streptomyces ansochromogenes

This is the first report that AdpA can simultaneously activate nikkomycin biosynthesis but repress oviedomycinynthesis in one strain and provide an effective strategy that is able to activate cryptic secondary metabolite gene clusters by genetic manipulation of global regulatory genes.

Eliciting the silent lucensomycin biosynthetic pathway in Streptomyces cyanogenus S136 via manipulation of the global regulatory gene adpA

It is hypothesized that the global transcriptional factor AdpA, due to its highly degenerate operator sequence, could be used to upregulate the expression of silent BGCs and uncovered the entire gene cluster for lucensomycin biosynthesis, that remained elusive for five decades until now.

Discovery of a Cryptic Antifungal Compound from Streptomyces albus J1074 Using High-Throughput Elicitor Screens.

A chemogenetic high-throughput screening approach to discover small molecule elicitors of silent biosynthetic gene clusters and successfully used this approach to activate a silent gene cluster in Streptomyces albus J1074, revealing the cytotoxins etoposide and ivermectin as potent inducers.

Genome Mining of Streptomyces sp. Tü 6176: Characterization of the Nataxazole Biosynthesis Pathway

Bioinformatic analysis of the genome of this organism predicts the presence of 38 putative secondary‐metabolite biosynthesis gene clusters, including those involved in the biosynthesis of AJI9561 and its derivative nataxazole, the antibiotic hygromycin B, and ionophores enterobactin and coelibactin.



The candicidin gene cluster from Streptomyces griseus IMRU 3570.

Of particular interest is the presence of the CanP1 loading domain (the first described as responsible for the activation of an aromatic starter unit) and the polypeptide CanP3 (carrying modules for the formation of five out of seven conjugated double bonds).

Activation and silencing of secondary metabolites in Streptomyces albus and Streptomyces lividans after transformation with cosmids containing the thienamycin gene cluster from Streptomyces cattleya

This work revealed the potential of S. albus to produce many others secondary metabolites normally obtained from plants, including compounds of medical relevance as dihydro-β-agarofuran and of interest in perfume industry as β-patchoulene, suggesting that it might be an alternative model for their industrial production.

Identification and characterization of an indigoidine-like gene for a blue pigment biosynthesis in Streptomyces aureofaciens CCM 3239

The results indicate the involvement of the bpsA gene in biosynthesis of the indigoidine blue pigment in S. aureofaciens CCM 3239, found to produce an extracellular blue pigment with identical properties as indigoidsine.

Identification of a bioactive 51-membered macrolide complex by activation of a silent polyketide synthase in Streptomyces ambofaciens

There is a constant need for new and improved drugs to combat infectious diseases, cancer, and other major life-threatening conditions. The recent development of genomics-guided approaches for novel

Identification of a cluster of genes that directs desferrioxamine biosynthesis in Streptomyces coelicolor M145.

The desferrioxamine biosynthetic pathway belongs to a new and rapidly emerging family of pathways for siderophore biosynthesis, widely distributed across diverse species of bacteria, which is biochemically distinct from the better known nonribosomal peptide synthetase (NRPS) pathway.

Streptomyces and Saccharopolyspora hosts for heterologous expression of secondary metabolite gene clusters

  • R. H. Baltz
  • Biology, Engineering
    Journal of Industrial Microbiology & Biotechnology
  • 2010
The results of several laboratory and industrial production strains used for heterologous production of secondary metabolite pathways are discussed, and the pros and cons of using various Streptomyces and one Saccharopolyspora strain forheterologous expression are discussed.

Characterization of Indigoidine Biosynthetic Genes in Erwinia chrysanthemi and Role of This Blue Pigment in Pathogenicity

In the plant-pathogenic bacterium Erwinia chrysanthemi, indigoidine production conferred an increased resistance to oxidative stress, indicating that indigoidsine may protect the bacteria against the reactive oxygen species generated during the plant defense response.

Identification of Two Genes from Streptomyces argillaceus Encoding Glycosyltransferases Involved in Transfer of a Disaccharide during Biosynthesis of the Antitumor Drug Mithramycin

It is proposed that the glycosyltransferases encoded by mtmGI and mtmGII are responsible for forming and transferring the disaccharide during mithramycin biosynthesis.

Cross‐regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor

A network of functional interactions among regulators that govern production of antibiotics and other secondary metabolites in S. coelicolor are revealed, which suggest that revision of the currently prevalent view of higher‐level versus pathway‐specific regulation of secondary metabolism in Streptomyces species is warranted.