Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target.

Abstract

RATIONALE Pulmonary hypertension is a fatal disease; however, its pathogenesis still remains to be elucidated. Thrombin-activatable fibrinolysis inhibitor (TAFI) is synthesized by the liver and inhibits fibrinolysis. Plasma TAFI levels are significantly increased in chronic thromboembolic pulmonary hypertension (CTEPH) patients. OBJECTIVE To determine the role of activated TAFI (TAFIa) in the development of CTEPH. METHODS AND RESULTS Immunostaining showed that TAFI and its binding partner thrombomodulin (TM) were highly expressed in the pulmonary arteries (PAs) and thrombus in patients with CTEPH. Moreover, plasma levels of TAFIa were increased 10-fold in CTEPH patients compared with controls. In mice, chronic hypoxia caused a 25-fold increase in plasma levels of TAFIa with increased plasma levels of thrombin and TM, which led to thrombus formation in PA, vascular remodeling, and pulmonary hypertension. Consistently, plasma clot lysis time was positively correlated with plasma TAFIa levels in mice. Additionally, overexpression of TAFIa caused organized thrombus with multiple obstruction of PA flow and reduced survival rate under hypoxia in mice. Bone marrow transplantation showed that circulating plasma TAFI from the liver, not in the bone marrow, was activated locally in PA endothelial cells through interactions with thrombin and TM. Mechanistic experiments demonstrated that TAFIa increased PA endothelial permeability, smooth muscle cell proliferation, and monocyte/macrophage activation. Importantly, TAFIa inhibitor and peroxisome proliferator-activated receptor-α agonists significantly reduced TAFIa and ameliorated animal models of pulmonary hypertension in mice and rats. CONCLUSIONS These results indicate that TAFIa could be a novel biomarker and realistic therapeutic target of CTEPH.

DOI: 10.1161/CIRCRESAHA.117.310640

Cite this paper

@article{Satoh2017ActivatedTP, title={Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target.}, author={Taijyu Satoh and Kimio Satoh and Nobuhiro Yaoita and Nobuhiro Kikuchi and Junichi Omura and Ryo Kurosawa and Kazuhiko Numano and Elias Al-Mamun and Mohammad Abdul Hai Siddique and Shinichiro Sunamura and Masamichi Nogi and Kota Suzuki and Satoshi Miyata and John Morser and Hiroaki Shimokawa}, journal={Circulation research}, year={2017}, volume={120 8}, pages={1246-1262} }