Actionable diagnosis of neuroleptospirosis by next-generation sequencing.

  title={Actionable diagnosis of neuroleptospirosis by next-generation sequencing.},
  author={Michael Wilson and Samia N. Naccache and Erik Samayoa and Mark J Biagtan and Hiba Bashir and Guixia Yu and Shahriar M. Salamat and S. Somasekar and Scot Federman and Steve A. Miller and Robert A Sokolic and Elizabeth N Garabedian and Fabio Candotti and Rebecca H Buckley and Kurt D. Reed and Teresa L Meyer and Christine M Seroogy and Renee Y Galloway and Sheryl L. Henderson and James E Gern and Joseph L. DeRisi and Charles Y Chiu},
  journal={The New England journal of medicine},
  volume={370 25},
A 14-year-old boy with severe combined immunodeficiency presented three times to a medical facility over a period of 4 months with fever and headache that progressed to hydrocephalus and status epilepticus necessitating a medically induced coma. Diagnostic workup including brain biopsy was unrevealing. Unbiased next-generation sequencing of the cerebrospinal fluid identified 475 of 3,063,784 sequence reads (0.016%) corresponding to leptospira infection. Clinical assays for leptospirosis were… CONTINUE READING


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