Action on bovine alpha s1-casein of cardosins A and B, aspartic proteinases from the flowers of the cardoon Cynara cardunculus L.

@article{RamalhoSantos1996ActionOB,
  title={Action on bovine alpha s1-casein of cardosins A and B, aspartic proteinases from the flowers of the cardoon Cynara cardunculus L.},
  author={Miguel Ramalho-Santos and Paula Ver{\'i}ssimo and Carlos Faro and Euclides Vieyras Pires},
  journal={Biochimica et biophysica acta},
  year={1996},
  volume={1297 1},
  pages={83-9}
}
The cleavage of purified bovine alpha s1-casein separately by cardosin A and cardosin B, two distinct milk-clotting aspartic proteinases (APs) present in the stigmas of the plant Cynara cardunculus L., was studied. Casein digestion peptides were separated either by SDS-PAGE or by reverse-phase HPLC, and their N-terminal amino acid sequences were subsequently determined by automated Edman degradation, thus identifying the cleavage sites. Results showed that both enzymes exert a similar but… CONTINUE READING

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