Action of the active metabolites of tiazofurin and ribavirin on purified IMP dehydrogenase.

@article{Yamada1988ActionOT,
  title={Action of the active metabolites of tiazofurin and ribavirin on purified IMP dehydrogenase.},
  author={Yasukazu Yamada and Yutaka Natsumeda and George Weber},
  journal={Biochemistry},
  year={1988},
  volume={27 6},
  pages={2193-6}
}
The inhibitory mechanisms of ribavirin 5'-monophosphate (RMP) and thiazole-4-carboxamide adenine dinucleotide (TAD), the active forms of the antimetabolites ribavirin and tiazofurin, were investigated in IMP dehydrogenase purified to homogeneity from rat hepatoma 3924A. The hepatoma IMP dehydrogenase has a tetrameric structure with a subunit molecular weight of 60,000. For the substrates IMP and NAD+, Km's were 23 and 65 microM, respectively. Product-inhibition patterns showed an ordered Bi-Bi… CONTINUE READING

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