Action of internal pronase on the f-channel kinetics in the rabbit SA node.

@article{Barbuti1999ActionOI,
  title={Action of internal pronase on the f-channel kinetics in the rabbit SA node.},
  author={Andrea Barbuti and Mirko Baruscotti and Claudia Altomare and Anna Moroni and Dario DiFrancesco},
  journal={The Journal of physiology},
  year={1999},
  volume={520 Pt 3},
  pages={737-44}
}
1. The hyperpolarization-activated If current was recorded in inside-out macropatches from sino-atrial (SA) node myocytes during exposure of their intracellular side to pronase, in an attempt to verify if cytoplasmic f-channel domains are involved in both voltage- and cAMP-dependent gating. 2. Superfusion with pronase caused a quick, dramatic acceleration of channel opening upon hyperpolarization and slowing, rapidly progressing into full blockade, of channel closing upon depolarization; these… CONTINUE READING