Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined trifunctional scaffoldin.

@article{Firobe2005ActionOD,
  title={Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined trifunctional scaffoldin.},
  author={H P Fi{\'e}robe and Florence Mingardon and Adva Mechaly and Anne B{\'e}la{\"i}ch and Marco Tulio Rinc{\'o}n and Sandrine Pag{\`e}s and Raphael Lamed and Chantal Tardif and J. -P. Bela{\"i}ch and Edward A Bayer},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 16},
  pages={
          16325-34
        }
}
In recent work, we reported the self-assembly of a comprehensive set of defined "bifunctional" chimeric cellulosomes. Each complex contained the following: (i) a chimeric scaffoldin possessing a cellulose-binding module and two cohesins of divergent specificity and (ii) two cellulases, each bearing a dockerin complementary to one of the divergent cohesins. This approach allowed the controlled integration of desired enzymes into a multiprotein complex of predetermined stoichiometry and topology… CONTINUE READING

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Trifunctional Designer Cellulosomes

M. Hammel, H. P. Fierobe, M. Czjzek, S. Finet, V. Receveur-Brechot
  • J. Biol. Chem
  • 2004

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