Action of Beryllium Ions on Primary Cultures of Swine Cells

  title={Action of Beryllium Ions on Primary Cultures of Swine Cells},
  author={Maria Vegni Talluri and Viviana Guiggiani},
SUMMARYIn cells cultured in vitro Beryllium exerts a damaging action on all the mitotic phases. In high concentrations (1×10–3M) such action is stronger on lymphocytes than on kidney cells.The mitotic index as well as mitotic morphology is impaired. The abnormalities are mainly stickiness, anaphasic bridges and migration of undivided chromosomes. No action on the achromatic spindle was observed.With the employed technique many chromatids breakages are also obtained; these do not appear to be… 


Dichloromethane induced transformation of virus-infected Fischer rat and Syrian hamster embryo cens and induced chromosomal aberrations, but not mutation or DNA damage, in rodent cens in vitro; conflcting results were reported for the induction of sister chromatid exchanges in Chinese hamster cells.

Effects of coal combustion products and metal compounds on sister chromatid exchange (SCE) in a macrophagelike cell line

  • O. Andersen
  • Biology
    Environmental health perspectives
  • 1983
The results demonstrated that the use of phagocytizing cells for assessment of genotoxic effects of particles offers a useful system, since the particles can be investigated directly and the extracted particles still increased SCE, but less than unextracted particles.

Interference of Beryllium with enzyme induction in rat liver.

Beryllium-induced ultrastructural changes in intact and regenerating liver.

Light microscope studies revealed liver necrosis in animals surviving 48 hours, and abolition of mitoses in animals treated 20 or 16 hours before death.

A Survey of Metal-induced Mutagenicity in Vitro and in Vivo

A survey of the literature shows that organic and inorganic compounds of 53 metals have been assayed for genotoxic effects in vitro and in vivo and that a wide range of compounds of the different metals is positive in at least one of the short-term tests.

Beryllium chloride-induced oxidative DNA damage and alteration in the expression patterns of DNA repair-related genes.

Mice exposed to beryllium chloride at an oral dose of 23mg/kg for seven consecutive days exhibited a significant increase in the level of DNA-strand breaking and micronuclei formation as detected by a bone marrow standard comet assay andmicronucleus test, which confirm the involvement of oxidative stress in the induction of bone marrow genetic damage after exposure to beriescence chloride.

Metabolism and possible health effects of aluminum.

A hypothetical model is presented for the metabolism of aluminum, based on documented direct observations of Al3+ and analogies from other ions, and it is proposed that an accumulation may take place at a subcellular level without any significant increase in the corresponding tissue concentration.

Recent Advances in Understanding the Biomolecular Basis of Chronic Beryllium Disease: A Review

In this review we summarize the work conducted over the past decade that has advanced our knowledge of pulmonary diseases associated with exposure to beryllium that has provided a molecular-based



Action of Beryllium on Cells cultivated in vitro; Effect on Mitosis

The effect of beryllium (sulphate) acting for one to four days on tissue cultures of connective tissue and skeletal muscles (chick embryos) and the final concentration varied from M/400 to M/106.


1. Cytochemical studies of the intracellular distribution of alkaline phosphatase in rat liver have been made, using a fractionation procedure recently developed in this laboratory (8) and a similar

Inhibition of Tertiary Calcium Phosphate Crystal Growth with Low Concentrations of Beryllium

It is indicated that extremely low concentrations of beryllium ion (1 part per 100,000,000) can limit crystal growth, which may be performed in vivo by critical concentrations of naturally occurring ions, such as citrate, polyphosphate, or some unknown substance(s).