Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells

@article{Gottlieb1993ActinMP,
  title={Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells},
  author={T. Gottlieb and I. Ivanov and M. Adesnik and D. Sabatini},
  journal={The Journal of Cell Biology},
  year={1993},
  volume={120},
  pages={695 - 710}
}
Treatment with cytochalasin D, a drug that acts by inducing the depolymerization of the actin cytoskeleton, selectively blocked endocytosis of membrane bound and fluid phase markers from the apical surface of polarized MDCK cells without affecting the uptake from the basolateral surface. Thus, in MDCK cell transformants that express the VSV G protein, cytochalasin blocked the internalization of an anti-G mAb bound to apical G molecules, but did not reduce the uptake of antibody bound to the… Expand
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TLDR
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Actin is required for endocytosis at the apical surface of Madin-Darby canine kidney cells where ARF6 and clathrin regulate the actin cytoskeleton.
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Observations indicate that concomitant to actin recruitment, the apical clathrin endocytic system is deeply involved in the morphology of the apicals plasma membrane. Expand
The effects of cytochalasin D and phorbol myristate acetate on the apical endocytosis of ricin in polarised Caco-2 cells.
TLDR
Stimulation of apical endocytosis of ricin by phorbol 12-myristate 13-acetate showed the characteristics of being mediated by protein kinase C, was not due to an effect on ricin movement along the microvillar surface, and may be explained by increases in formation and pinching off of clathrin coated pits at the apical cell surface. Expand
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Both Microtubules and Actin Filaments Are Required for Efficient Postendocytotic Traffic of the Polymeric Immunoglobulin Receptor in Polarized Madin-Darby Canine Kidney Cells*
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Results suggest that in addition to microtubules, efficient postendocytic traffic in polarized epithelial cells also requires actin filaments. Expand
Evidence for Apical Endocytosis in Polarized Hepatic Cells: Phosphoinositide 3-Kinase Inhibitors Lead to the Lysosomal Accumulation of Resident Apical Plasma Membrane Proteins
TLDR
A long-held but unproven assumption that lysosomes are the final destination of apical membrane proteins in hepatocytes is confirmed, and the hypothesis that SAC is not an apical endosome is confirmed. Expand
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Actin filaments facilitate two steps of endocytosis.
TLDR
It is shown that actin filaments are involved in two steps of the degradative pathway, and that microtubules are required at a stage in between, and for the first time the sequential involvement of actin Filaments and microtubule along one intracellular membrane trafficking pathway is indicated. Expand
Actin microfilaments et al.--the many components, effectors and regulators of epithelial cell endocytosis.
The aim of this review is to introduce the advances made over the past several years regarding the participation of actin and actin-associated proteins in clathrin-mediated endocytosis in simple cellExpand
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ENDOCYTOSIS IN CHANG LIVER CELLS: Quantitation by Sucrose-3H Uptake and Inhibition by Cytochalasin B
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