Actin disruption inhibits bombesin stimulation of focal adhesion kinase (pp125FAK) in prostate carcinoma.

  title={Actin disruption inhibits bombesin stimulation of focal adhesion kinase (pp125FAK) in prostate carcinoma.},
  author={Mark D. Duncan and John W. Harmon and L Duncan},
  journal={The Journal of surgical research},
  volume={63 1},
UNLABELLED Jasplakinolide is a member of a new class of antitumor agents targeting the actin cytoskeleton with activity against prostate cancer. Focal adhesion kinase (FAK) is an actin-associated mediator of mitogenic peptides. We hypothesized that the neuropeptide bombesin would activate FAR in prostate carcinoma, and that disruption of the actin network would block FAK activation and inhibit cell growth. METHODS PC-3 human prostate carcinoma cells were exposed to 50-200 nM jasplakinolide… Expand
Focal adhesion kinase is required for bombesin-induced prostate cancer cell motility
Observations point towards a critical role for FAK in the action of BN on PC-3 cell motility in prostate cancer. Expand
Bombesin stimulates the motility of human prostate‐carcinoma cells through tyrosine phosphorylation of focal adhesion kinase and of integrin‐associated proteins
It is shown that bombsin enhances the migration of androgen‐independent PCa cells (PC‐3) in vitro, while not affecting their adhesion to extracellular matrix proteins, and tyrosine phosphorylation of pp125FAK and integrin‐associated proteins may play an important role in bombesin signaling, triggering the processes of PCa cell motility and invasion. Expand
Characterization of the activities of actin-affecting drugs on tumor cell migration.
The present work aims to develop a multi-assay strategy for screening actin-affecting drugs with anti-migratory potentials and shows concordant drug-induced effects on actin polymerization occurring in vitro in test tubes and within whole cells. Expand
Cucurbitacin E targets proliferating endothelia.
CuE preferentially potently inhibits proliferating human endothelia compared to quiescent cells in vitro, and at low concentrations, cucurbitacin E may potently inhibit vascular proliferation by disrupting actin. Expand
Jasplakinolide: an actin-specific reagent that promotes actin polymerization.
  • A. Holzinger
  • Biology, Medicine
  • Methods in molecular biology
  • 2009
In this chapter, the visualization of jasplaklinolide effects by different fluorescent and transmission electron microscopic methods is described, and alternatives are given here as competitive binding capacities ofjasplakinolide and phalloidin make the detection of actin filaments by fluorescently labeled phalloids problematic. Expand
Alteration of actin organization by jaspamide inhibits ruffling, but not phagocytosis or oxidative burst, in HL-60 cells and human monocytes.
Jaspamide disrupts the actin cytoskeleton of normal and malignant mammalian cells with no significant effect on phagocytic activity and may, therefore, be considered as a novel therapeutic agent. Expand
Clinical significance of FAK expression in human neoplasia.
The purpose of this article is a comprehensive review of the existing data on FAK expression and signaling and their clinical significance in human malignancy. Expand
Targeting actin remodeling profiles for the detection and management of urothelial cancers--a perspective for bladder cancer research.
  • J. Rao
  • Biology, Medicine
  • Frontiers in bioscience : a journal and virtual library
  • 2002
The overall hypothesis is that actin alterations are progressive and that distinctive actin remodeling profiles are associated with different stages of cancer development and progression, thereby providing an actin-based paradigm for individualized monitoring and intervention of human bladder cancer. Expand
Alterations of the actin polymerization status as an apoptotic morphological effector in HL‐60 cells
The results show that the regulation of actin polymerization is an important apoptotic morphological effector, whereas the alterations of the act in polymerization status by chemicals have profound effects not only on altering the morphology of apoptotic cells, but on apoptosis induction in HL‐60 cells as well. Expand
Jasplakinolide: interaction with radiation and hyperthermia in human prostate carcinoma and Lewis lung carcinoma
Jasplakinolide is an interesting new anticancer agent for which further study both as an antic cancer agent and in combined modality regimens is warranted. Expand