Actin-binding proteins from Drosophila embryos: a complex network of interacting proteins detected by F-actin affinity chromatography

Abstract

By using F-actin affinity chromatography columns to select proteins solely by their ability to bind to actin filaments, we have identified and partially purified greater than 40 proteins from early Drosophila embryos. These proteins represent approximately 0.5% of the total protein present in soluble cell extracts, and 2 mg are obtained by chromatography of… (More)

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