Actin assembly induced by polylysine beads or purified phagosomes: quantitation by a new flow cytometry assay.


BACKGROUND Actin assembly on biological membranes is a poorly understood process. We have previously shown that phagosomal membranes could induce actin assembly in the presence of thymosin beta4 (an actin sequestering protein that inhibits nonspecific nucleation), via the barbed ends of actin filaments. METHODS Here, we have developed an in vitro system… (More)


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