Actin antibody: the specific visualization of actin filaments in non-muscle cells.

@article{Lazarides1974ActinAT,
  title={Actin antibody: the specific visualization of actin filaments in non-muscle cells.},
  author={Elias Lazarides and Klaus -Josef Weber},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1974},
  volume={71 6},
  pages={2268-72}
}
Actin purified from mouse fibroblasts by sodium dodecyl sulfate gel electrophoresis was used as antigen to obtain an antibody in rabbits. The elicited antibody was shown to be specific for actin as judged by immunodiffusion and complement fixation against partially purified mouse fibroblast actin and highly purified chicken muscle actin. The antibody was used in indirect immunofluorescence to demonstrate by fluorescence light microscopy the distribution and pattern of actin-containing filaments… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 168 extracted citations

Actin visualization at a glance.

Journal of cell science • 2017
View 1 Excerpt

References

Publications referenced by this paper.
Showing 1-5 of 5 references

Cytoplasmic actin and myosin and cell

T. D. Pollard, Weihing, R Il.
1973
View 4 Excerpts
Highly Influenced

Actin and myosin and cell movement.

CRC critical reviews in biochemistry • 1974

Cold Spring Harbor Snymp

A. J. Spudich
Quant . Biol . • 1972

Cold Spring Harbor Synmp

D. Brav
Qziant . Biol . • 1972

& ' Mazia , 1 ) . ( 1970 )

L. Wilson, J. Bryan, A. Ruby
Proc . Nat . Acad . Sci . USA

Similar Papers

Loading similar papers…