ActP controls copper homeostasis in Rhizobium leguminosarum bv. viciae and Sinorhizobium meliloti preventing low pH‐induced copper toxicity

@article{Reeve2002ActPCC,
  title={ActP controls copper homeostasis in Rhizobium leguminosarum bv. viciae and Sinorhizobium meliloti preventing low pH‐induced copper toxicity},
  author={W. Reeve and R. Tiwari and Neetin B. Kale and M. Dilworth and A. Glenn},
  journal={Molecular Microbiology},
  year={2002},
  volume={43}
}
Two ‘calcium‐irreparable’ acid‐sensitive mutants were identified after mutagenizing Rhizobium leguminosarum bv. viciae and Sinorhizobium meliloti with Tn5. Each mutant contains a single copy of the transposon which, inserted within the actP gene, prevents expression of a P‐type ATPase that belongs to the CPx heavy metal‐transporting subfamily. Here, we show that both actP‐knockout mutants show sensitivity to copper; omission of this heavy metal from low pH‐buffered media restores acid tolerance… Expand
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