Acrosome reaction and Ca²⁺ imaging in single human spermatozoa: new regulatory roles of [Ca²⁺]i.

@article{SnchezCrdenas2014AcrosomeRA,
  title={Acrosome reaction and Ca²⁺ imaging in single human spermatozoa: new regulatory roles of [Ca²⁺]i.},
  author={Claudia S{\'a}nchez-C{\'a}rdenas and Martha Rocio Serv{\'i}n-Vences and O. San Jos{\'e} and Claudia Lydia Trevi{\~n}o and Arturo Hern{\'a}ndez-Cruz and Alberto Darszon},
  journal={Biology of reproduction},
  year={2014},
  volume={91 3},
  pages={67}
}
The spermatozoa acrosome reaction (AR) is essential for mammalian fertilization. Few methods allow visualization of AR in real time together with Ca²⁺ imaging. Here, we show that FM4-64, a fluorescent dye used to follow exocytosis, reliably reports AR progression induced by ionomycin and progesterone in human spermatozoa. FM4-64 clearly delimits the… CONTINUE READING