Acrosomal biogenesis in human globozoospermia: immunocytochemical, ultrastructural and proteomic studies.

@article{AlvarezSed2012AcrosomalBI,
  title={Acrosomal biogenesis in human globozoospermia: immunocytochemical, ultrastructural and proteomic studies.},
  author={Cristian Alvarez Sed{\'o} and Vanesa Y. Rawe and H{\'e}ctor E. Chemes},
  journal={Human reproduction},
  year={2012},
  volume={27 7},
  pages={
          1912-21
        }
}
BACKGROUND Acrosome biogenesis is a key event in sperm differentiation that depends on the proper interaction between the Golgi complex and the nuclear envelope of early spermatids. We studied the development, structure and biochemical characteristics of human acrosomes in germ cells and spermatozoa from testicular biopsies and semen samples of fertile men and patients with acrosomeless spermatozoa (globozoospermia). A set of proteins collectively known as the perinuclear theca (PT), which has… 
View on PubMed
academic.oup.com
41 Citations
Highly Influential Citations
3
Background Citations
22
Methods Citations
1
Results Citations
2

Figures and Tables from this paper

41 Citations

Citation Type

Citation Type

New insights into sperm with total globozoospermia: Increased fatty acid oxidation and centrin1 alteration
TLDR
Sperm with globozoospermia showed an increased susceptibility to non-enzymatic fatty acid oxidation that could be, at least in part, responsible for fertilization failure in intracytoplasmic sperm injection.
Comparative testicular transcriptome of wild type and globozoospermic Dpy19l2 knock out mice
TLDR
It is shown that the DPY19L2 protein is located in the inner nuclear membrane of spermatids during spermiogenesis and that it is necessary to anchor the acrosome to the nucleus thus performing a function similar to that realized by Sun proteins within the LINC-complex (Linker of Nucleoskeleton and Cytoskeleton).
Association between total globozoospermia and sperm chromatin defects
TLDR
Assessment and compare the degree of sperm protamine deficiency and DNA fragmentation in large population of infertile men with total globozoospermia with 22 fertile individuals using chromomycin A3 and TUNEL assay respectively shows that mean of sperm concentration and percentage of sperm motility were significantly lower, while percentages of sperm abnormal morphology, protamine‐deficient and DNA‐fragmented spermatozoa were significantly higher in infertiles men with globozoopermia.
Centriolar defects, centrin 1 alterations, and FISH studies in human spermatozoa of a male partner of a couple that produces aneuploid embryos in natural and artificial fertilization
TLDR
A paternal contribution to sperm aneuploidies cannot be excluded since the man of a couple who obtained trisomic embryos with natural and assisted fertilization showed altered morphology, immature centriolar adjunct, presence of evident distal centriole, scarce presence of centrin 1, and high aneuPLoidy frequency.
Comparative profiling of the sperm proteome
TLDR
This review outlines recent achievements of sperm proteomics and exemplifies the usefulness of gel‐based surveys by outlining the comparative analysis of abnormal spermatozoa in globozoospermia.
Decreased Expression of KIFC1 in Human Testes with Globozoospermic Defects
Globozoospermia is a rare (prevalence of <0.1%) but severe male infertility condition. In our previous study, we found that robust KIFC1 immunostaining was detected in the human elongating/elongated…
Ultrastructural analysis of testicular tissue and sperm by transmission and scanning electron microscopy.
  • H. Chemes
  • Biology
    Methods in molecular biology
  • 2013
TLDR
Various ultrastructural techniques that are now available for basic or translational research in reproductive biology and reproductive medicine are described, including TEM, ultrastructureural immunocytochemistry, ultrastsructural histochemistry, and SEM.
Haploid male germ cells-the Grand Central Station of protein transport.
TLDR
It is believed that the continuous and spatiotemporally restrained nature of spermiogenesis provides an outstanding model system to identify, and de-code, cytoskeletal elements and transport mechanisms of relevance to multiple tissues.
Chapter 3 – The Spermatozoon
Clinical and Structural Features of Sperm Head Vacuoles in Men Included in the In Vitro Fertilization Programme
TLDR
The ultrastructural study revealed that sperm head vacuoles are large nuclear indentations of various sizes and positions, packed with membranous material organized in membrane whorls (MW).
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 61 REFERENCES
Lack of acrosome formation in mice lacking a Golgi protein, GOPC
  • R. Yao, C. Ito, +7 authors T. Noda
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2002
TLDR
Golgi-associated PDZ- and coiled-coil motif-containing protein (GOPC) provides important clues to understanding the mechanisms underlying spermatogenesis, and the GOPC-deficient mouse may be a unique and valuable model for human globozoospermia.
Distribution and possible role of perinuclear theca proteins during bovine spermiogenesis
TLDR
It is suggested that the perinuclear theca proteins play an instrumental role in the attachment, spreading, and binding of the acrosome onto the nucleus of spermatids.
Membrane trafficking machinery components associated with the mammalian acrosome during spermiogenesis.
TLDR
During murine spermiogenesis, Golgi antigens are detected in the acrosome until the late cap-phase spermatids, but are not found in testicular spermatozoa, suggesting that Golgi-acrosome flow may be relatively unselective, with Golgi residents retrieved before spermiation is complete.
The major subacrosomal occupant of bull spermatozoa is a novel histone H2B variant associated with the forming acrosome during spermiogenesis.
TLDR
This study identifies the major SDS-insoluble PT protein, which is localized to the subacrosomal layer of bovine spermatozoa and cloned by immunoscreening a bull testicular cDNA library and postulated roles of SubH2Bv in acrosomes assembly and acrosome-nuclear docking.
Failure of differentiation of the nuclear-perinuclear skeletal complex in the round-headed human spermatozoa.
  • D. Escalier
  • Biology
    The International journal of developmental biology
  • 1990
TLDR
Data obtained suggest that round-headed sperm head anomalies result from a failure of differentiation of the sperm-specific skeletal complex related to the nucleus and the acrosome spreading over the nucleus, the nuclear elongation and the post-acrosomal sheath formation are dependent on such nuclear-perinuclear differentiations.
Biogenesis of sperm perinuclear theca and its role in sperm functional competence and fertilization.
Human acrosome biogenesis: immunodetection of proacrosin in primary spermatocytes and of its partitioning pattern during meiosis.
TLDR
Data reveal proacrosin biosynthesis during diploid and haploid phases of human spermatogenesis and the proACrosin partitioning pattern during meiosis and the single labelled area observed in early sperMatids was found to increase considerably in size during spermiogenesis.
The Golgi apparatus segregates from the lysosomal/acrosomal vesicle during rhesus spermiogenesis: structural alterations.
TLDR
It is found that the Golgi apparatus is cast off from the acrosomal vesicle and migrates toward the sperm tail in elongated spermatids, and is undetectable in mature ejaculated spermatozoa.
Morphogenesis of Round‐Headed Human Spermatozoa Lacking Acrosomes in a Case of Severe Teratozoospermia
TLDR
Some ultrastructural findings, not reported up to day, are described in the testicular biopsy specimen from an infertile man with this type of severe teratozoospermia, to provide information of value to a better understanding of the basic mechanism underlying this types of pathomorphogenesis.
Protein composition of the perinuclear theca of bull spermatozoa.
TLDR
Evidence is provided that the protein composition of the bull perinuclear theca is complex: at least six major polypeptides make up this cytoskeletal element.
...
1
2
3
4
5
...