Acridine orange-induced precipitation of mouse testicular sperm cell DNA reveals new patterns of chromatin structure.

@article{Evenson1990AcridineOP,
  title={Acridine orange-induced precipitation of mouse testicular sperm cell DNA reveals new patterns of chromatin structure.},
  author={Donald P. Evenson and Zbigniew Darzynkiewicz},
  journal={Experimental cell research},
  year={1990},
  volume={187 2},
  pages={328-34}
}
Precipitate resulting from interaction between certain intercalators, such as acridine orange (AO), and nucleic acids can be detected by electron microscopy. Formation of precipitate in nuclei of live cells is modulated by chromatin structure. Susceptibility of in situ DNA to precipitation was studied in mouse testicular germ cells during various stages of sperm maturation. DNA in round spermatid chromatin, similar to somatic cell euchromatin, was rather resistant to precipitation; the electron… CONTINUE READING