Acridine orange fluorescence techniques as alternatives to traditional Giemsa staining for the diagnosis of malaria in developing countries.

@article{Lowe1996AcridineOF,
  title={Acridine orange fluorescence techniques as alternatives to traditional Giemsa staining for the diagnosis of malaria in developing countries.},
  author={Brett Lowe and N K Jeffa and Liguo New and Court Pedersen and K Engbaek and Kevin Marsh},
  journal={Transactions of the Royal Society of Tropical Medicine and Hygiene},
  year={1996},
  volume={90 1},
  pages={34-6}
}
Traditional Giemsa-stained thick blood films were compared with 2 fluorescence microscopy techniques, acridine orange (AO) staining of thin blood films and the quantitative buffy coat (QBC) method, for the microscopical diagnosis of malaria. Of 200 samples examined, 141 were positive by Giemsa staining, 146 by AO and 137 by QBC. Overall sensitivities for the 2 fluorescence techniques compared to Giemsa staining were good: AO 97.9% and QBC 93.6%. However, with parasitaemias < 100/microL the QBC… CONTINUE READING

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