Acquisition of uncharacterized sequences from Candidatus liberibacter, an unculturable bacterium, using an improved genomic walking method.

@article{Lin2008AcquisitionOU,
  title={Acquisition of uncharacterized sequences from Candidatus liberibacter, an unculturable bacterium, using an improved genomic walking method.},
  author={Hong Lin and Harshavardhan Doddapaneni and Xianjing Bai and Jiqiang Yao and Xiaolong Zhao and Edwin L. Civerolo},
  journal={Molecular and cellular probes},
  year={2008},
  volume={22 1},
  pages={
          30-7
        }
}
An effective PCR-based genomic walking approach is described to discover previously unknown flanking genomic DNA sequences from Candidatus Liberibacter asiaticus, an unculturable, phloem-limited bacterium. Using this technique, 8564bp of new DNA sequences were obtained from three genomic loci; tufB-secE-nusG-rplKAJL-rpoBC gene cluster, omp gene (outer membrane protein, Omp) and 16/23S rRNA gene in Ca. L. asiaticus. These, together with publicly available Ca. Liberibacter sequences, are… CONTINUE READING

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