Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation.

@article{Satomura2013AcquisitionOT,
  title={Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation.},
  author={Atsushi Satomura and Yoshiaki Katsuyama and Natsuko Miura and Kouichi Kuroda and Ayako Tomio and Takeshi Bamba and Eiichiro Fukusaki and Mitsuyoshi Ueda},
  journal={Biotechnology progress},
  year={2013},
  volume={29 5},
  pages={1116-23}
}
A thermotolerant Saccharomyces cerevisiae yeast strain, YK60-1, was bred from a parental strain, MT8-1, via stepwise adaptation. YK60-1 grew at 40°C, a temperature at which MT8-1 could not grow at all. YK60-1 exhibited faster growth than MT8-1 at 30°C. To investigate the mechanisms how MT8-1 acquired thermotolerance, DNA microarray analysis was performed. The analysis revealed the induction of stress-responsive genes such as those encoding heat shock proteins and trehalose biosynthetic enzymes… CONTINUE READING