Acquisition, culture, and phenotyping of synovial fibroblasts.

  title={Acquisition, culture, and phenotyping of synovial fibroblasts.},
  author={Sanna Rosengren and David L. Boyle and Gary S. Firestein},
  journal={Methods in molecular medicine},
The study of fibroblast-like synoviocytes (FLS) has yielded important insights into the pathogenic mechanisms of rheumatoid arthritis. FLS can be cultured from synovial tissue obtained at joint replacement surgery, synovectomy, or synovial biopsy. After collagenase digestion, adherent cells consist mainly of synovial fibroblasts and synovial macrophages. Proliferating FLS are enriched by repeated passage and comprise >95% of cells by passage 3. Because of cell senescence, use of FLS lines after… CONTINUE READING

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