Acquired defects of glycogen synthase activity in cultured human skeletal muscle cells: influence of high glucose and insulin levels.

@article{Henry1996AcquiredDO,
  title={Acquired defects of glycogen synthase activity in cultured human skeletal muscle cells: influence of high glucose and insulin levels.},
  author={Robert R. Henry and Theodore P. Ciaraldi and S. A. Jegaraya Mudaliar and Liane Abrams and Svetlana E Nikoulina},
  journal={Diabetes},
  year={1996},
  volume={45 4},
  pages={400-7}
}
To determine whether defects of muscle glycogen synthase (GS) activity can be acquired by exposure to elevated glucose or insulin levels, human skeletal muscle cells obtained by needle biopsy from normal control subjects were grown in culture for 4-6 weeks followed by 4 days of fusion and differentiation in media containing either normal (5.5 mmol/l glucose and 22 pmol/l insulin) or increased concentrations of glucose (20 mmol/l), insulin (30 micromol/l), or both. After fusion in normal media… CONTINUE READING