Acidocalcisomes and a vacuolar H+-pyrophosphatase in malaria parasites.

  title={Acidocalcisomes and a vacuolar H+-pyrophosphatase in malaria parasites.},
  author={Norma Marchesini and Shuhong Luo and Claudia O. Rodrigues and Silvia N. J. Moreno and Roberto Docampo},
  journal={The Biochemical journal},
  volume={347 Pt 1},
Plasmodium berghei trophozoites were loaded with the fluorescent calcium indicator, fura-2 acetoxymethyl ester, to measure their intracellular Ca(2+) concentration ([Ca(2+)](i)). [Ca(2+)](i) was increased in the presence of the sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase inhibitor, thapsigargin. Trophozoites also possess a significant amount of Ca(2+) stored in an acidic compartment. This was indicated by: (1) the increase in [Ca(2+)](i) induced by bafilomycin A(1), nigericin, monensin, or… CONTINUE READING