Acidification of the male reproductive tract by a proton pumping(H+)-ATPase

@article{Breton1996AcidificationOT,
  title={Acidification of the male reproductive tract by a proton pumping(H+)-ATPase},
  author={Sylvie Breton and Peter J. S. Smith and B Lui and Dennis Brown},
  journal={Nature Medicine},
  year={1996},
  volume={2},
  pages={470-472}
}
An acidic luminal pH (ref. 1–3) is involved in sperm maturation, and in maintaining sperm in an immotile state in the epididymis and vas deferens2,4–6. Neutralization by prostatic fluid is one of a complex series of events that triggers sperm motility2,7,8. Failure of the acidification mechanism might, therefore, result in poor sperm maturation, premature motility and infertility. We have shown that a vacuolar (H+)–ATPase is expressed at high levels on the luminal plasma membrane of specialized… 
Proton secretion in the male reproductive tract: involvement of Cl--independent HCO-3 transport.
TLDR
The inhibition by SITS observed under both normal and Cl--free conditions indicates that a Cl-/HCO-3 exchanger is not involved and that an alternative H CO-3 transporter participates in proton secretion in the proximal vas deferens.
Role of V-ATPase-rich cells in acidification of the male reproductive tract.
TLDR
Manipulation of luminal pH might, therefore, provide a point of intervention for the regulation of male fertility and it is possible that some cases of unexplained male infertility might result from defective acidification, resulting either from pathological states or potentially from environmental factors that may inhibit proton secretory pathways.
Tetanus toxin-mediated cleavage of cellubrevin inhibits proton secretion in the male reproductive tract.
TLDR
The results suggest that H(+)-ATPase is actively endocytose and exocytosed in proton-secreting cells of the epididymis and vas deferens and that net proton secretion requires the participation of the v-SNARE cellubrevin.
Regulation of luminal acidification in the male reproductive tract via cell–cell crosstalk
TLDR
A complex interplay between the different cell types present in the epithelium leads to activation of the luminal acidifying capacity of the epididymis, a process that is crucial for sperm maturation and storage.
Establishment of cell-cell cross talk in the epididymis: control of luminal acidification.
TLDR
This review describes how epididymal epithelial cells work in a concerted manner, together with spermatozoa, to establish and maintain this acidic luminal environment.
Distribution of the Vacuolar H+ATPase Along the Rat and Human Male Reproductive Tract1
TLDR
It is concluded that the vacuolar H+ATPase is highly expressed in epithelial cells of most segments of the male reproductive tract in rat and man, where it may be involved in H+ secretion and/or intracellular processing of the material endocytosed from the luminal fluid or destined to be secreted by exocytosis.
Cadmium Inhibits Vacuolar H+ATPase-Mediated Acidification in the Rat Epididymis1
TLDR
Alkalinization of the tubule fluid in the epididymis and vas deferens of Cd-treated rats may result from the loss of functional H+ATPase enzyme in the cell apical domain as well as from a direct inhibition of H+ ATPase function by Cd.
Regulation of vacuolar proton pumping ATPase-dependent luminal acidification in the epididymis.
TLDR
In the present paper, selected aspects of V-ATPase regulation in clear cells are described and potential pathologies associated with mutations of some of the V- ATPase subunits are discussed.
Immunolocalization of AE2 anion exchanger in rat and mouse epididymis.
TLDR
A recently described SDS antigen unmasking treatment is used to localize the Cl/HCO(3) exchanger AE2 in rat and mouse epididymis and confirms the identity of the AE2 protein, which may participate in bicarbonate reabsorption and luminal acidification, and/or may be involved in intracellular pH homeostasis of epithelial cells of the male reproductive tract.
Postnatal development of H+ATPase (proton-pump)-rich cells in rat epididymis
TLDR
It is demonstrated that differentiated subpopulations of proton-secreting epithelial cells appear early during epididymal development, and that the induction of H+ATPase in these cells occurs prior to sexual maturation.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 21 REFERENCES
A plasma membrane proton ATPase in specialized cells of rat epididymis.
Acidification of the luminal fluid in the epididymis is believed to play an important role in sperm maturation. Previous studies have shown that specialized cells in the epithelium lining the
Cellular organization of urinary acidification.
TLDR
Structural-function studies of CO2 stimulation of H+ secretion by alpha-CA cells indicate that the secretion rate (JH) correlates with apical membrane area and numbers of RSPs, and the view that R SPs represent arrays of transmembrane channels and studs represent catalytic units of H- pumps is supported by quantitative considerations but remains to be proven.
Localization of a proton-pumping ATPase in rat kidney.
TLDR
It is demonstrated that subpopulations of cortical intercalated cells have opposite polarities of an H+ATPase, consistent with the presence of both proton- and bicarbonate-secreting cells, and suggested a role for the H+ ATPase in acid/base regulation or H+ transport in segments other than the collecting duct and the proximal tubule.
Inhibition of bovine spermatozoa by caudal epididymal fluid: II. Interaction of pH and a quiescence factor.
TLDR
It is determined that the pH of bovine CE fluid and of CE semen is approximately 5.8, and that the motility of CE sperm in undiluted CE fluid increases as the pH is elevated, suggesting that the acidity of CE fluid may play a physiological role in the maintenance of sperm quiescence.
Expression of carbonic anhydrase isoenzymes IV and II in rat epididymal duct.
TLDR
The results imply that in rat epididymis both bicarbonate reabsorption and proton secretion are involved in epidodymal fluid acidification, and suggest that CA IV is involved in bic carbonate re absorption mainly occurring in the corpus epididycumidis.
Absence of H(+)-ATPase in cortical collecting tubules of a patient with Sjogren's syndrome and distal renal tubular acidosis.
TLDR
It is demonstrated that the basis for the proton secretory defect in some patients with distal renal tubular acidosis is likely the absence of H(+)-ATPase in the intercalated cells.
An H+-ATPase in opposite plasma membrane domains in kidney epithelial cell subpopulations
TLDR
This is the first direct demonstration of neighbouring epithelial cells maintaining opposite polarities of a transport protein, and it is found that some cortical collecting duct intercalated cells have apical plasma membrane proton pumps, whereas others have basolateral pumps.
Direct evaluation of acidification by rat testis and epididymis: role of carbonic anhydrase.
The present experiments have employed microelectrode techniques (pH and PCO2) and microcalorimetry (total CO2 concentration) to define parameters of acidification in specific structures of the rat
Intracellular pH regulates bovine sperm motility and protein phosphorylation.
TLDR
It is reported that sperm pHi, measured with the fluorescent pH probe carboxyfluorescein, increases by approximately 0.4 units in response to either pH elevation or dilution of the fluid that initiate sperm motility.
Epididymal epithelium: Its contribution to the formation of a luminal fluid microenvironment
TLDR
This review focusses on current understanding of the contributions of the epididymal epithelium to the formation of a specialized luminal fluid microenvironment.
...
1
2
3
...