• Chemistry, Medicine
  • Published in
    The Journal of biological…
    1989

Acidification and ion permeabilities of highly purified rat liver endosomes.

@article{Fuchs1989AcidificationAI,
  title={Acidification and ion permeabilities of highly purified rat liver endosomes.},
  author={Roland J. Fuchs and Philippe M{\^a}le and Ira Mellman},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 4},
  pages={
          2212-20
        }
}
While it is well established that acidic pH in endosomes plays a critical role in mediating the orderly traffic of receptors and ligands during endocytosis, little is known about the bioenergetics or regulation of endosome acidification. Using highly enriched fractions of rat liver endosomes prepared by free flow electrophoresis and sucrose density gradient centrifugation, we have analyzed the mechanism of ATP-dependent acidification and ion permeability properties of the endosomal membrane… CONTINUE READING

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