Acidic amino acid binding sites in mammalian neuronal membranes: their characteristics and relationship to synaptic receptors

@article{Foster1984AcidicAA,
  title={Acidic amino acid binding sites in mammalian neuronal membranes: their characteristics and relationship to synaptic receptors},
  author={Alan Carl Foster and Graham E. Fagg},
  journal={Brain Research Reviews},
  year={1984},
  volume={7},
  pages={103-164}
}
This review summarizes studies designed to label and characterize mammalian synaptic receptors for glutamate, aspartate and related acidic amino acids using in vitro ligand binding techniques. The binding properties of the 3 major ligands employed--L-[3H]glutamate, L-[3H]aspartate and [3H]kainate--are described in terms of their kinetics, the influence of ions, pharmacology, molecular nature, localization and physiological/pharmacological function. In addition, the binding characteristics are… 
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TLDR
It is made that the d-aspartate-insensitive binding site, reported in this work, might be related to a quisqualate-type receptor which becomes predominantly exposed following depolarization and might exert then a modulation of neuronal transmission in the rat striatum.
The binding of acidic amino acids to snail, Helix aspersa, periosophagic ring membranes reveals a single high-affinity glutamate/kainate site
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Brain synaptic membranes are enriched in high affinity L-glutamic acid binding sites which have a number of the pharmacologic characteristics of the receptor for this excitatory amino acid, and it was shown that this high affmity glutamate binding activity of synaptic membranes is associated with a small molecular weight glycoprotein.
Effects of protein- and membrane-modifying agents on the binding of l-[3H]glutamate to cerebellar synaptic membranes
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Chloride and calcium ions reveal a pharmacologically distinct population of L-glutamate binding sites in synaptic membranes: correspondence between biochemical and electrophysiological data
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TLDR
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Complex Binding of l‐[3H]Glutamate to Hippocampal Synaptic Membranes in the Absence of Sodium
Abstract: Specific binding of L‐[3H]glutamate was investigated with a thoroughly washed synaptic membrane preparation from rat hippocampal formation, a region of brain densely innervated by
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TLDR
There are at least four distinct classes of 3H-L-glutamate binding sites which differ in their anatomical distribution, pharmacological profile and regulation by ions, and a third site may represent the KA receptor and a fourth binding site does not conform to present receptor classifications.
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TLDR
The results indicate that [3H]L-glutamate labels a single class of sites that can be resolved into subpopulations by agonists and antagonists and provide additional evidence of excitatory amino acid receptor heterogeneity.
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TLDR
In studies of subcellular fractionation of the brain, it was found that crude synaptosomal membrane preparations were most enriched in specific [3H]kainic acid binding, and the specified binding was localized to grey matter in the brain.
Cysteine Sulfinic Acid in the Central Nervous System: Specific Binding of [35S]Cysteic Acid to Cortical Synaptic Membranes‐An Investigation of Possible Binding Sites for Cysteine Sulfinic Acid
TLDR
Results indicate the existence of an Na+‐independent specific binding site for cysteic acid in the synaptic membrane of rat cerebral cortex, which may be different from that for glutamate.
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