Acid stabilization of Bacillus licheniformis alpha amylase through introduction of mutations

@article{Liu2008AcidSO,
  title={Acid stabilization of Bacillus licheniformis alpha amylase through introduction of mutations},
  author={Yi-han Liu and Fu-ping Lu and Yu Li and Jian-ling Wang and Chen Ni Gao},
  journal={Applied Microbiology and Biotechnology},
  year={2008},
  volume={80},
  pages={795-803}
}
This paper provided further understanding of the relationships between acid resistance and structural features of different mutants in Bacillus licheniformis alpha amylase (BLA) due to the changes of two crucial positions Leu134 and Ser320. In order to investigate effect of the two positions on the acid stability, we described the detailed characterization of wild-type and the single mutants L134R and S320A as well as the double mutant L134R/S320A. The highest k cat /Km with pH 4.5… CONTINUE READING

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Protein engineering of bacterial alpha-amylases.

Biochimica et biophysica acta • 2000
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