Acid phospholipase A1 requiring phospholipids or Triton X-100 in the cytosol of cultured cells.

Abstract

A new type of soluble phospholipase A1 [EC 3.1.1.32] was found in the supernatant fraction (cytosol) obtained by centrifugation at 105,000 x g for 90 min of homogenates in 0.25 M sucrose of FL cells and several other cultured cells (L-132 cells, Vero cells, and BEK-1 cells). Phospholipase A1 in FL cell cytosol showed activity only in the presence of a non… (More)

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