Acid-labile subunit of human insulin-like growth factor-binding protein complex: measurement, molecular, and clinical evaluation.

@article{Khosravi1997AcidlabileSO,
  title={Acid-labile subunit of human insulin-like growth factor-binding protein complex: measurement, molecular, and clinical evaluation.},
  author={Mohammad Javad Khosravi and Anastasia Diamandi and Jehangir Mistry and R K Gopala Krishna and Amit Kumar Khare},
  journal={The Journal of clinical endocrinology and metabolism},
  year={1997},
  volume={82 12},
  pages={3944-51}
}
Although the acid-labile subunit (ALS) of the approximately 150-kDa insulin-like growth factor (IGF)-binding protein (IGFBP) complex was described over a decade ago, details of ALS physiology have remained largely uncertain. We evaluated antibodies to synthetic human ALS and constructed a noncompetitive ALS enzyme-linked immunosorbent assay. Whereas uncomplexed ALS is directly measured, determination of total levels required sample pretreatment with SDS, which was found to optimally dissociate… CONTINUE READING