Acid- and base-induced conformational transitions of equinatoxin II.

@article{Poklar2001AcidAB,
  title={Acid- and base-induced conformational transitions of equinatoxin II.},
  author={N Poklar and Joe Voelker and Gregor Anderluh and Peter Ma{\vc}ek and T V Chalikia},
  journal={Biophysical chemistry},
  year={2001},
  volume={90 2},
  pages={103-21}
}
We have investigated the acid- and base-induced conformational transitions of equinatoxin II (EqTxII), a pore-forming protein, by a combination of CD-spectroscopy, ultrasonic velocimetry, high precision densimetry, viscometry, gel electrophoresis, and hemolytic activity assays. Between pH 7 and 2, EqTxII does not exhibit any significant structural changes. Below pH 2, EqTxII undergoes a native-to-partially unfolded transition with a concomitant loss of its rigid tertiary structure and the… CONTINUE READING