Acid Extrusion from Human Spermatozoa Is Mediated by Flagellar Voltage-Gated Proton Channel

@article{Lishko2010AcidEF,
  title={Acid Extrusion from Human Spermatozoa Is Mediated by Flagellar Voltage-Gated Proton Channel},
  author={Polina V. Lishko and Inna L. Botchkina and Andriy Fedorenko and Yuriy Kirichok},
  journal={Cell},
  year={2010},
  volume={140},
  pages={327-337}
}
Human spermatozoa are quiescent in the male reproductive system and must undergo activation once introduced into the female reproductive tract. This process is known to require alkalinization of sperm cytoplasm, but the mechanism responsible for transmembrane proton extrusion has remained unknown because of the inability to measure membrane conductance in human sperm. Here, by successfully patch clamping human spermatozoa, we show that proton channel Hv1 is their dominant proton conductance… Expand
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