Acetylglutamate synthase from Neurospora crassa: structure and regulation of expression

@article{Yu1996AcetylglutamateSF,
  title={Acetylglutamate synthase from Neurospora crassa: structure and regulation of expression},
  author={Y. G. Yu and Gloria E. Turner and Richard L. Weiss},
  journal={Molecular Microbiology},
  year={1996},
  volume={22}
}
A DNA clone which complemented an arg‐14 mutation of Neurospora crassa was isolated by sib selection from a cosmid library (pMOcosX). Southern and restriction‐fragment‐length polymorphism (RFLP) analysis confirmed that the cloned DNA contained the arg‐14 gene. The arg‐14 gene was identified as the structural gene for acetylglutamate synthase by immunodepletion of enzyme activity with antibodies prepared against an arg‐14 fusion protein and by the thermal instability of acetylglutamate synthase… Expand
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